Translational_Unit
Csy4-H29A

Part:BBa_K2615007:Design

Designed by: Anyi Li   Group: iGEM18_OUC-China   (2018-09-07)


Csy4-H29A, the No.5 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

We designed this part by point mutation. We changed the CAC(encoding His ) to GCG(encoding Ara) on the 29th site based on wild type Csy4.

Source

Science Museum No.105，College of Marine Life Sciences, Ocean University of China

References

[http://science.sciencemag.org/content/329/5997/1355 Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence- and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997):1355-1358.]